By default, Tophat reports only "accepted_hits.sam". Is there any way to get a similar output with the unaligned reads ? Bowtie accepts the --un option to specify an output file, but Tophat does not recognize this option. Thanks for any input !

asked 30 Jun '10, 08:32

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sandmann
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Simon Andrews in this thread (http://seqanswers.com/forums/showthread.php?t=6847) has a perl script that can use the original fastq and outputed sam files to find the unaligned reads. I've found no mention of a --un feature for Tophat.

link

answered 08 Dec '11, 12:11

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shershman
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Asked: 30 Jun '10, 08:32

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Last updated: 08 Dec '11, 12:11

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