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Hi! I am absolutely new to this new NGS/bioinformatics era. I am working with RNAseq and currently I have performed my alignments using Novoalign. According to my co-workers they look alright. I was told the next step is to sort my data using Sam tools. I am using the code: samtools view -bS (input file.sam) | samtools sort - (outputfile.bam) I have read the manual to see what is it that I am doing, but still is not clear to me. Could anybody help me in understanding what the sort samtools command does to my newly generated alignment? Thanks! John |
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Asked: 01 Dec '11, 10:56
Seen: 114 times
Last updated: 01 Dec '11, 10:56
